Epitope mapping of an anti-diacylglycerol kinase delta monoclonal antibody DdMab-1

Characterisation of anti-IgG monoclonal antibody A57H by epitope mapping.

Monoclonal antibodies have proved powerful tools in serological techniques and as probes for elucidating the antigenic determinants of macromolecules and cells. However an inherent problem of these reagents is the phenomenon of assay restriction, where a given antibody may perform well in some assays, whilst being poor or ineffective in others [I] . Thus stringent evaluation of a monoclonal ant...

Epitope mapping and use of anti-idiotypic antibodies to the L6 monoclonal anticarcinoma antibody.

Mouse monoclonal anti-idiotypic antibodies (anti-ids) were raised against L6, a murine IgG2a monoclonal antibody specific for a cell surface antigen expressed by many human carcinomas. Ten distinct anti-ids were generated. Eight anti-ids were shown to inhibit the binding of L6 to its target antigen and were characterized in detail. The heavy and light chain variable region gene segments of the ...

Precise epitope determination of the anti-vimentin monoclonal antibody V9

Vimentin is a type III intermediate filament protein that is typically expressed in mesenchymal cells. Overexpression of vimentin is frequently observed in several types of cancer and is often associated with epithelial‑to‑mesenchymal transition. It was recently reported that the serum vimentin level is significantly elevated in colon and liver tumors. Therefore, a more sensitive vimentin detec...

Development of a Novel Inhibitory Chimeric Anti-HER2 Monoclonal Antibody

BER-H2: a new anti-Ki-1 (CD30) monoclonal antibody directed at a formol-resistant epitope.

The production and characterization of a monoclonal antibody (MoAb) designated Ber-H2, directed against a new epitope of the Ki-1 (CD30) antigen, are described. In comparison with the formerly reported Ki-1 MoAb whose reactivity with Hodgkin and Reed-Sternberg (H-RS) cells in frozen tissue sections is well-documented, the Ber-H2 MoAb showed new, important features: the labeling intensity of the...